Adipose stem cell-derived growth factors and proliferation of oral mucosa fibroblasts

doi:10.3969/j.issn.2095-4344.2013.32.006

Abstract

Abstract:

BACKGROUND: Adipose stem cells have been confirmed to promote the repair of soft tissue after damage, and the action mechanism is possibly related to the paracrine of adipose stem cells, that is adipose stem cells secrete a variety of cytokines, which may promote the restoration of damaged cells. However, little report addressed the types of adipose stem cells secreted factors, contents of each factor, and role in soft tissue repair after damage, especially oral mucosa.
OBJECTIVE: To observe the influence of vascular endothelial growth factor, platelet-derived growth factor, and basic fibroblast growth factor in adipose stem cell-conditioned medium on the proliferation of oral mucosa fibroblasts.
METHODS: Protein microarray analysis was used to analyze the contents of vascular endothelial growth factor, platelet-derived growth factor, and basic fibroblast growth factor in adipose stem cell-conditioned medium. CCK8 analysis was used to analyze the effects of adipose stem cell-conditioned medium with different concentrations of vascular endothelial growth factor, platelet-derived growth factor, and basic fibroblast growth factor (0, 1, 10, 20, 50 and 100 μg/L) or the neutralizing antibody of the three kinds of cytokines on the proliferation of oral mucosa fibroblast on 1, 2, 3, 4 and 5 days.
RESULTS AND CONCLUSION: High contents of vascular endothelial growth factor, platelet-derived growth factor, and basic fibroblast growth factor were contained in adipose stem cell-conditioned medium (signal value > 300). Adipose stem cell-conditioned medium could promote the proliferation of oral mucosa fibroblasts, wherein, the promotion effects of platelet-derived growth factor and basic fibroblast growth factor were very significant, and the peak changes could be observed with variation of cytokines concentrations. The optimal concentrations of platelet-derived growth factor and basic fibroblast growth factor were 50 μg/L and 1 μg/L, respectively (P ≤ 0.05). The neutralizing antibody of platelet-derived growth factor and basic fibroblast growth factor inhibited the promotion effect of adipose stem cell-conditioned medium. On the other hand, vascular endothelial growth factor had no significant effect on the proliferation of oral mucosa fibroblasts. Adipose stem cell-conditioned medium can promote the proliferation of oral mucosa fibroblasts, platelet-derived growth factor and basic fibroblast growth factor in adipose stem cell-conditioned medium have obvious promotion effects, which are dependent on the cytokine concentrations. Therefore, we should pay attention to choose the optimal concentrations of cytokines, thereby effectively promoting the proliferation of fibroblasts.

Key words: stem cells, adipose stem cells, multidirectional differentiation, conditioned medium, oral mucosa fibroblasts, vascular endothelial growth factor, platelet-derived growth factor, basic fibroblast growth factor, protein microarray analysis, National Natural Science Foundation of China, stem cell photographs-containing paper

CLC Number:

R394.2

Zhao Jia-jia, Hu Li, Liu Jia-rong, Gong Ni-ya, Chen Li-li. Adipose stem cell-derived growth factors and proliferation of oral mucosa fibroblasts[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(32): 5778-5784.

Figures/Tables 8

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